Gene Regulation

Research Interest

Head: Dr. habil. Helmut Bäumlein

We focus on the molecular dissection of genetic and epigenetic pathways that control sexual and asexual plant reproductive development. To answer our research questions we use genetic, molecular and imaging techniques in the model plant Arabidopsis (dicot), the parthenogenetic ´Salmon´wheat lines as well as the two apomictic species Hypericum and Boechera.  

Plant development is a recurrent process occurring in two distinct alternating generations, gametes-producing gametophyte and the spore-producing sporophyte. Plant gametes are contained in plant-specific haploid organisms known as the female (embryo sac) and male (pollen) gametophytes that are independently derived via meiotic reduction of the corresponding diploid spores. Fertilisation of haploid gametes (egg and sperm cells) leads to the development of the diploid embryo sporophyte. In flowering plants, a second fertilization event occurs in which the second female gamete, known as the homo-diploid central cell, is fertilized by an additional haploid sperm cell, leading to the development of triploid endosperm that nourishes the embryo. After early embryogenesis, the seed matures via synthesis of storage compounds as well as acquisition of desiccation tolerance and dormancy. Subsequently, the mature seed germinates into the whole plant sporophyte. 

While most plants reproduce sexually, some plant species in the wild reproduce asexually by a process known as apomixis. Both meiosis and egg fertilization are either avoided or circumvented during apomictic events such as apomeiosis and parthenogenesis, respectively; thereby, the offspring are the clonal replica of the mother plant.

Molecular dissection of these coordinated developmental events of both sexual and asexual plant reproduction is an exciting basic research field in plant biology and provides important information required for biotechnological applications such as food technology (e.g. cereal endosperm) and transfer of elite crop traits like heterosis via apomixis.

Publications

Author
Title
2019

Galla G, Siena L A, Ortiz J P A, Bäumlein H, Barcaccia G, Pessino S C, Bellucci M, Pupilli F:

A portion of the apomixis locus of Paspalum simplex is microsyntenic with an unstable chromosome segment highly conserved among Poaceae. Sci. Rep. 9 (2019) 3271. https://dx.doi.org/10.1038/s41598-019-39649-6

Rizzo P, Altschmied L, Stark P, Rutten T, Guendel A, Scharfenberg S, Franke K, Baeumlein H, Wessjohann L, Koch M, Borisjuk L, Sharbel T F:

Discovery of key regulators of dark glands development and hypericin biosynthesis in St. Johns wort (Hypericum perforatum). Plant Biotechnol. J. 17 (2019) 2299-2312. https://dx.doi.org/10.1111/pbi.13141

Tedeschi F, Rizzo P, Huong B, Czihal A, Rutten T, Altschmied L, Scharfenberg S, Grosse I, Becker C, Weigel D, Bäumlein H, Kuhlmann M:

EFFECTOR OF TRANSCRIPTION factors are novel plant-specific regulators associated with genomic DNA methylation in Arabidopsis. New Phytol. 221 (2019) 261-278. https://dx.doi.org/10.1111/nph.15439

2017

Tedeschi F, Rizzo P, Rutten T, Altschmied L, Bäumlein H:

RWP-RK domain-containing transcription factors control cell differentiation during female gametophyte development in Arabidopsis. New Phytol. 213 (2017) 1909–1924. https://dx.doi.org/10.1111/nph.14293

2016

Rizzo P:

Novel insights on female gametophyte development in the apomictic model species Boechera spp. and Hypericum spp. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2016) 182 pp.

Shah J N, Kirioukhova O, Pawar P, Tayyab M, Mateo J L, Johnston A J:

Depletion of key meiotic genes and transcriptome-wide abiotic stress reprogramming mark early preparatory events ahead of apomeiotic transition. Front. Plant Sci. 7 (2016) 1539. https://dx.doi.org/10.3389/Fpls.2016.01539

2015

Beie I:

RKD-Transkriptionsfaktoren als zentrale Regulatoren der Gametophyt-Entwicklung bei Arabidopsis thaliana. (Master Thesis) Magdeburg, Otto-von-Guericke-Universität (2015) 61 pp.

Huong B T M:

EFFECTOR OF TRANSCRIPTION (ET): Novel plant specific epigenetic regulators of reproductive processes act on DNA-methylation. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I Biowissenschaften (2015) 94 pp.

2014

Harshavardhan V T, Van Son L, Seiler C, Junker A, Weigelt-Fischer K, Klukas C, Altmann T, Sreenivasulu N, Bäumlein H, Kuhlmann M:

AtRD22 and AtUSPL1, members of the plant-specific BURP domain family involved in Arabidopsis thaliana drought tolerance. PLoS One 9 (2014) e110065. https://dx.doi.org/10.1371/journal.pone.0110065

2012

Ivanov R, Tiedemann J, Czihal A, Bäumlein H:

Transcriptional regulator AtET2 is required for the induction of dormancy during late seed development. J. Plant Physiol. 169 (2012) 501-508. https://dx.doi.org/10.1016/j.jplph.2011.11.017

Junker A, Bäumlein H:

Multifunctionality of the LEC1 transcription factor during plant development. Plant Signal. Behav. 7 (2012) 1718-1720. https://dx.doi.org/10.4161/psb.22365

Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi T M, Renou J P, Balzergue S, Viehover P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H:

Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana. Plant J. 71 (2012) 427-442. https://dx.doi.org/10.1111/j.1365-313X.2012.04999.x

Mönke G, Seifert M, Keilwagen J, Mohr M, Grosse I, Hähnel U, Junker A, Weisshaar B, Conrad U, Bäumlein H, Altschmied L:

Toward the identification and regulation of the Arabidopsis thaliana ABI3 regulon. Nucleic Acids Res. 40 (2012) 8240-8254. https://dx.doi.org/10.1093/nar/gks594

2011

Galla G, Barcaccia G, Schallau A, Puente Molins M, Bäumlein H, Sharbel T F:

The cytohistological basis of apospory in Hypericum perforatum L. Sex. Plant Reprod. 24 (2011) 47-61. https://dx.doi.org/10.1007/s00497-010-0147-7

Heckmann S, Lermontova I, Berckmans B, De Veylder L, Bäumlein H, Schubert I:

The E2F transcription factor family regulates CENH3 expression in Arabidopsis thaliana. Plant J. 68 (2011) 646-656. https://dx.doi.org/10.1111/j.1365-313X.2011.04715.x

Kirioukhova O, Johnston A J, Kleen D, Kägi C, Baskar R, Moore J M, Bäumlein H, Gross-Hardt R, Grossniklaus U:

Female gametophytic cell specification and seed development require the function of the putative Arabidopsis INCENP ortholog WYRD. Development 138 (2011) 3409-3420. https://dx.doi.org/10.1242/dev.060384

Kőszegi D, Johnston A J, Rutten T, Czihal A, Altschmied L, Kumlehn J, Wust S E, Kirioukhova O, Gheyselinck J, Grossniklaus U, Bäumlein H:

Members of the RKD transcription factor family induce an egg cell-like gene expression program. Plant J. 67 (2011) 280-291. https://dx.doi.org/10.1111/j.1365-313X.2011.04592.x

Le H D:

Functional characterization of Effector of Transcription (ET) in Arabidopsis thaliana. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Institut für Biologie (2011) 127 pp.

Lemcke R:

The RETINOBLASTOMA pathway controls reproductive development and genome integrity in Arabidopsis. (Bachelor Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2011) 43 pp.

Lermontova I, Koroleva O, Rutten T, Fuchs J, Schubert V, Moraes I, Köszegi D, Schubert I:

Knockdown of CENH3 in Arabidopsis reduces mitotic divisions and causes sterility by disturbed meiotic chromosome segregation. Plant J. 68 (2011) 40-50. https://dx.doi.org/10.1111/j.1365-313X.2011.04664.x

Mildner M:

Funktionelle Charakterisierung von Eizell-aktiven RKD-Faktoren in Arabidopsis thaliana. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2011) 128 pp.

2010

Aquea F, Johnston A J, Canon P, Grossniklaus U, Arce-Johnson P:

TRAUCO, a Trithorax-group gene homologue, is required for early embryogenesis in Arabidopsis thaliana. J. Exp. Bot. 61 (2010) 1215-1224. https://dx.doi.org/10.1093/jxb/erp396

Johnston A J, Kirioukhova O, Barrell P J, Rutten T, Moore J M, Baskar R, Grossniklaus U, Gruissem W:

Dosage-sensitive function of RETINOBLASTOMA RELATED and convergent epigenetic control are required during the Arabidopsis life cycle. PLoS Genet. 6 (2010) e1000988. https://dx.doi.org/10.1371/journal.pgen.1000988

Junker A, Hartmann A, Schreiber F, Bäumlein H:

An engineers view on regulation of seed development. Trends Plant Sci. 15 (2010) 303-307. https://dx.doi.org/10.1016/j.tplants.2010.03.005

Schallau A:

Identification and genetic analysis of the APOSPORY locus in Hypericum perforatum L. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Institut für Genetik (2010) 171 pp.

Schallau A, Arzenton F, Johnston A J, Hähnel U, Köszegi D, Blattner F, Altschmied L, Haberer G, Barcaccia G, Bäumlein H:

Identification and genetic analysis of the APOSPORY locus in Hypericum perforatum L. Plant J. 62 (2010) 773-784. https://dx.doi.org/10.1111/j.1365-313X.2010.04188.x

2009

Junker A:

Characterization of the LEAFY COTELYDON1 regulon: Transcription factor-controlled hormone cross-talk during somatic and zygotic embryogenesis. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg (2009) 171 pp.

Van Son L, Tiedemann J, Rutten T, Hillmer S, Hinz G, Zank T, Manteuffel R, Bäumlein H:

The BURP domain protein AtUSPL1 of Arabidopsis thaliana is destined to the protein storage vacuoles and overexpression of the cognate gene distorts seed development. Plant Mol. Biol. 71 (2009) 319-329. https://dx.doi.org/10.1007/s11103-009-9526-6

2008

Ivanov R, Tiedemann J, Czihal A, Schallau A, Diep L, Mock H-P, Claus B, Tewes A, Bäumlein H:

EFFECTOR OF TRANSCRIPTION2 is involved in xylem differentiation and includes a functional DNA single strand cutting domain. Dev. Biol. 313 (2008) 93-106. https://dx.doi.org/10.1016/j.ydbio.2007.09.061

Koszegi D:

RKD gene: a novel transcription factor family involved in the female gametophyte development of Arabidopsis and wheat. (PhD Thesis) Halle/S., Martin-Luther-Universität Halle-Wittenberg, Naturwissenschaftliche Fakultät I, Institut für Biologie, Institutsbereich Pflanzenphysiologie (2008) 105 pp.

Schallau A, Kakhovskaya I, Tewes A, Czihal A, Tiedemann J, Mohr M, Grosse I, Manteuffel R, Bäumlein H:

Phylogenetic footprints in fern spore- and seed-specific gene promoters. Plant J. 53 (2008) 414-424. https://dx.doi.org/10.1111/j.1365-313X.2007.03354.x

Takacs I, Koszegi D, Barnabas B:

cDNA library preparation from a single wheat kernel. Acta Biol. Cracov. Bot. 50 (2008) 105-109.

Tiedemann J, Rutten T, Mönke G, Vorwieger A, Rolletschek H, Meissner D, Milkowski C, Petereck S, Mock H-P, Zank T, Bäumlein H:

Dissection of a complex seed phenotype: novel insights of FUSCA3 regulated developmental processes. Dev. Biol. 317 (2008) 1-12. https://dx.doi.org/10.1016/j.ydbio.2008.01.034

2007

Barcaccia G, Bäumlein H, Sharbel T F:

Apomixis in St. Johns wort (Hypericum perforatum L.): an overview and glimpse towards the future. In: Hörandl E, Grossniklaus U, van Dijk P J, Sharbel T F (Eds.): Apomixis: evolution, mechanisms and perspectives. (Series: Regnum vegetabile, Vol. 147) Rugell, Liechtenstein: Gantner (2007) ISBN 978-3-906166-60-5, 259-280.

Kotak S, Vierling E, Bäumlein H, von Koskull-Döring P:

A novel transcriptional cascade regulating expression of heat stress proteins during seed development of Arabidopsis. Plant Cell 19 (2007) 182-195. https://dx.doi.org/10.1105/tpc.106.048165

Matzk F, Prodanovic S, Czihal A, Tiedemann J, Arzenton F, Blattner F R, Kumlehn J, Altschmied L, Schubert I, Johnston A, Grossniklaus U, Bäumlein H:

Genetic control of apomixis: preliminary lessons from Poa, Hypericum and wheat egg cells. In: Hörandl E, Grossniklaus U, van Dijk P J, Sharbel T F (Eds.): Apomixis: evolution, mechanisms and perspectives. (Series: Regnum vegetabile, Vol. 147) Rugell, Liechtenstein: Gantner (2007) ISBN 978-3-906166-60-5, 159-166.

Mönke G, Linh T M, Conrad U, Hähnel U, Altschmied L, Mohr M, Grosse I, Vorwieger A, Bäumlein H, Weisshaar B, Viehöver P:

GABI-ARABIDO-SEED: Wie steuern Transkriptionsfaktoren die Samenentwicklung bei Pflanzen? GenomXPress Sonderausgabe März (2007) 16.

Perovic D, Tiffin P, Douchkov D, Bäumlein H, Graner A:

An integrated approach for the comparative analysis of a multigene family: The nicotianamine synthase genes of barley. Funct. Integr. Genomics 7 (2007) 169-179. https://dx.doi.org/10.1007/s10142-006-0040-5

Vorwieger A, Gryczka C, Czihal A, Douchkov D, Tiedemann J, Mock H-P, Jakoby M, Weisshaar B, Saalbach I, Bäumlein H:

Iron assimilation and transcription factor controlled synthesis of riboflavin in plants. Planta 226 (2007) 147-158. https://dx.doi.org/10.1007/s00425-006-0476-9

Wang H Y, Klatte M, Jakoby M, Bäumlein H, Weisshaar B, Bauer P:

Iron deficiency-mediated stress regulation of four subgroup Ib BHLH genes in Arabidopsis thaliana. Planta 226 (2007) 897-908. https://dx.doi.org/10.1007/s00425-007-0535-x

2006

Zimmermann G, Bäumlein H, Mock H-P, Himmelbach A, Schweizer P:

The multigene family encoding germin-like proteins of barley. Regulation and function in basal host resistance1[W][OA]. Plant Physiol. 142 (2006) 181-192. https://dx.doi.org/10.1104/pp.106.083824